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Recombinant protein production in prokaryotic systems

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8c00-b481-a468-831a80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Use of prokaryotic expression systems offers an economical method to achieve production of large amounts of recombinant protein. The Facility provides technical support for production of recombinant protein in bacteria. The core maintains a repository of inducible bacterial expression vector technologies. This collection of expression vectors allows for fusion of epitope tags (alone or in combination) to the protein of interest (GOI), including GST (Glutathione-S-transferase), 6 histidines (6His), SUMO, FLAG, or maltose binding protein (MBP) at the NH3- or COOH-terminus of a protein. High level protein expression (analytical or preparative scale) is achieved by IPTG induction of T7/T5 RNA polymerases in high-performance Epicuran Coli (e.g. Rosetta (DE3))." Services include: optimization of soluble protein expression and preparative production or recombinant proteins.
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/protein-expression-and-libraries-facility/policies-and-user-fees
  4. Service Provided by
    Protein Expression Facility (Wistar)
  5. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/protein-expression-facility/services/recombinant-protein-produc-0
  6. Related Technique
    Recombinant protein production
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-28T14:20:39.055-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:54:07.041-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016