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Recombinant protein purification

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8c04-4c3c-a468-831a80000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The Facility provides analytical and preparative scale, one- or two-step purification of recombinant proteins expressed in bacteria and baculovirus infected insect cells. The expectation is to provide highly purified recombinant proteins for use in structure-function studies, crystallographic efforts, assay development for high-throughput small molecule screening, custom antibody productions, and peptide identification of macromolecular protein complexes. Since most recombinant protein expression in bacterial and baculovirus systems takes advantage of epitope tags for monitoring expression, proteins are purified from soluble extracts using appropriate affinity matrices (e.g., Ni2+-/Co2+-agarose, GSH-sepharose, amylose-agarose, anti-FLAG/HA agarose) that allow for selection of specific tags (e.g. 6His, GST). When necessary, a second purification step (e.g., affinity, size exclusion chromatography, etc) is included to ensure maximum purity of the recombinant protein. The core cleaves epitope tags, when requested, and the recombinant protein will be further purified from tags and proteases. Purified proteins are dialyzed into appropriate buffers and concentrated to desired concentrations."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/protein-expression-and-libraries-facility/policies-and-user-fees
  4. Service Provided by
    Protein Expression Facility (Wistar)
  5. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/protein-expression-facility/services/recombinant-protein-purifica
  6. Related Technique
    Protein purification
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-28T14:23:22.838-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:54:05.547-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016