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Metabolite assay in blood and tissues

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000140-f452-0efa-91c7-0c6080000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    The MC@CHOP offers a variety of analytical techniques to quantitate stable isotope enrichment (13C, 2H, 15N) and to perform metabolic profiling. Our analytical repertoire includes the following: Stable Isotope Analysis, including: -- Stable isotope enrichment (13C, 15N, 2H, 18O) in glucose, amino acids, NH3, urea, tricarboxylic acid cycle intermediates, fatty acids and other intermediates of metabolism -- 13CO2 enrichment in blood and/or expired air for studies of oxidative metabolism -- Doubly labelled water (2H2O/H218O ratio) for studies of metabolic rate in a freely moving organism -- Measurement - both in vivo and in vitro - of flux through metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, the urea cycle, protein synthesis/degradation. -- Metabolomics profiling including, measurements of amino acids, organic acids, NAD+, NADH, NADP+, NADPH, adenine nucleotides (AMP, ADP, ATP), Pi, triglycerides, free fatty acids, glucose, putrescine, pyridoxines, pyridoxal, protein, etc. -- Carnitine / Acylcarnitines levels and profiling both in blood and tissue extracts -- Measurement of neurotransmitters and neuromodulator levels and profiling -- Measurement of D- or L- forms of amino acids and their levels both in physiological fluid and tissue extracts. -- Measurement of selected drug and/or drug metabolites as well as determining the impact of a given drug on metabolome and fluxome. -- Measurement of signaling compounds such as, cAMP in various tissues.
  3. Related Resource
    Mass spec
  4. Service Provided by
    Metabolomic Core (CHOP)
  5. Related Technique
    Mass spectrometry
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    ggrant (Gregory Grant)
  3. created
    2013-09-06T13:31:09.513-04:00
  4. creator
    fcoldren
  5. modified
    2019-10-09T12:36:31.207-04:00
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016