Drd5-/-

eagle-i ID

Resource Type

Properties

1. Resource Description

   D5DR KO mice have targeted disruption of the gene for dopamine receptor D5 and were "generated by mating either heterozygotes or homozygotes, and genotype was determined by PCR." "A neo was inserted in reverse orientation to disrupt the reading frame within the coding region. A stop codon was engineered into the proximal neo linker to prematurely truncate the transcript subsequent to Gly-190 in the second extracellular loop. RT-PCR verified presence of the recombinant transcript in mutant brains. Immunohistochemistry using peptides from the region downstream of the stop codon showed that mutants did not produce the corresponding protein." "We thank [...] D. Sibley (National Institutes of Health) for providing stock for the [...] DA receptor KO mouse lines."

2. Additional Name

   D5 KO

3. Additional Name

   D5DR KO

4. Related Publication or Documentation

   Redundant catecholamine signaling consolidates fear memory via phospholipase C.

5. Website(s)

   http://www.informatics.jax.org/allele/key/35666

6. Parental Strain Name

   C57BL/6 background

7. Biological process studied

   memory

8. Genetic Alteration(s)

   Drd5[tm1Sib]

9. Location

   Steven Thomas Laboratory