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Drd5-/-

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000141-efe1-45bb-91c7-0c6080000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    D5DR KO mice have targeted disruption of the gene for dopamine receptor D5 and were "generated by mating either heterozygotes or homozygotes, and genotype was determined by PCR." "A neo was inserted in reverse orientation to disrupt the reading frame within the coding region. A stop codon was engineered into the proximal neo linker to prematurely truncate the transcript subsequent to Gly-190 in the second extracellular loop. RT-PCR verified presence of the recombinant transcript in mutant brains. Immunohistochemistry using peptides from the region downstream of the stop codon showed that mutants did not produce the corresponding protein." "We thank [...] D. Sibley (National Institutes of Health) for providing stock for the [...] DA receptor KO mouse lines."
  2. Additional Name
    D5 KO
  3. Additional Name
    D5DR KO
  4. Related Publication or Documentation
    Redundant catecholamine signaling consolidates fear memory via phospholipase C.
  5. Website(s)
    http://www.informatics.jax.org/allele/key/35666
  6. Parental Strain Name
    C57BL/6 background
  7. Biological process studied
    memory
  8. Genetic Alteration(s)
    Drd5[tm1Sib]
  9. Location
    Steven Thomas Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-10-25T09:54:35.973-04:00
  4. creator
    apernot (Anne Claire Pernot)
  5. modified
    2013-10-29T10:57:32.004-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016