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Rosa26-floxed stop zsGreen

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000141-f016-3324-91c7-0c6080000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    "Ai6 mice hemizygous for this Rosa-CAG-LSL-ZsGreen1-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced green fluorescent protein (ZsGreen1). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of ZsGreen1. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ZsGreen1 expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai6 mice do not express ZsGreen1 prior to introduction of Cre recombinase and ZsGreen1 expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Bright fluorescence is observed mainly in cell bodies. Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if so desired. Similarly, the attB/attP-flanked selection cassette may be removed by introduction of the site-specific bacteriophage PhiC31 integrase if so desired. These Ai6 mice are useful as a Cre reporter strain; expressing the enhanced green fluorescent protein, ZsGreen1, following Cre-mediated recombination. For characterization information, see images at the Allen Institute for Brain Science website (Ai6 images)."
  2. Related Disease
    diabetes mellitus
  3. Related Disease
    obesity
  4. Website(s)
    http://jaxmice.jax.org/strain/007906.html
  5. Website(s)
    http://www.informatics.jax.org/allele/MGI:3809522
  6. Biological process studied
    neurological system process
  7. Genetic Alteration(s)
    Gt(ROSA)26Sor[tm6(CAG-ZsGreen1)Hze]
  8. Location
    Steven Thomas Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-10-25T11:12:34.447-04:00
  4. creator
    apernot (Anne Claire Pernot)
  5. modified
    2013-11-08T11:20:19.768-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016