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KA1R-Cre Tg

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000142-1a62-ad88-91c7-0c6080000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    "Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These Cre-expressing mice may be useful in neurological studies involving hippocampus learning and memory, area CA3, and pyramidal cells." "The transgenic constuct consisted of the Grik4 promoter and exons 1 and 2 adjoined to the cre recombinase coding region. The Grik4 promoter was shown to drive expression in the CA3 pyramidal cell layer by staining of a mouse also harboring a lacZ reporter transgene that is expressed upon cre mediated recombination."
  2. Website(s)
    http://jaxmice.jax.org/strain/006474.html
  3. Biological process studied
    Behavior
  4. Biological process studied
    memory
  5. Biological process studied
    neurological system process
  6. Genetic Alteration(s)
    Tg(Grik4-cre)G32-4Stl
  7. Location
    Steven Thomas Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-11-02T15:56:44.125-04:00
  4. creator
    apernot (Anne Claire Pernot)
  5. modified
    2013-11-08T11:09:49.844-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016