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Rosa26-floxed stop tdTomato

eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    "Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if so desired. Similarly, the attB/attP-flanked selection cassette may be removed by introduction of the site-specific bacteriophage PhiC31 integrase if so desired. These Ai9 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination. The Allen Institute for Brain Science (AIBS) website has specific characterization information for several Cre Driver and Cre Reporter lines. Please see their website for images of AIBS experiments performed with all lines. Of note, Ai9 mice may also be available on a C57BL/6J congenic background (see Stock No. 007909)."
  2. Related Disease
    diabetes mellitus
  3. Related Disease
  4. Website(s)
  5. Biological process studied
    neurological system process
  6. Genetic Alteration(s)
  7. Location
    Steven Thomas Laboratory
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
  3. created
  4. creator
    apernot (Anne Claire Pernot)
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016