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tetO-tdTomato/SypGFP Tg

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000142-1a72-596d-91c7-0c6080000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    "Hemizygous TRE-Bi-SG-T line 1.1 transgenic mice are viable and fertile, with no reported phenotypic abnormalities. The TRE-Bi-SG-T transgene has Myc-tagged tdTomato and full-length mouse synaptophysin/mut4EGFP fusion protein (Syp-GFP) expression under the control of the bi-directional tet-responsive promoter (tetO or TRE). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), tdTomato and Syp-GFP fusion protein expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. In tTA(-dox) or rtTA(+dox)-expressing cells, tdTomato expression is directed to the entire cell, while GFP expression is directed to the synapse/synaptic vesicle. The donating investigator reports that direct GFP fluorescence and direct tdTomato fluorescence may be visualized in these mice when tTA is present/dox is absent. In addition, the three Myc epitopes at the C-terminus of tdTomato allow fluorescent signal enhancement via immunofluorescence if needed. These TRE-Bi-SG-T transgenic mice are useful to generate mutant mice for Tet-Off/Tet-On and/or fluorescent protein applications. These TRE-Bi-SG-T transgenic mice may be used to design a tripartite system to express fluorescently tagged synaptic proteins with both spatial and temporal control. When bred with floxed-STOP-tTA mice (ROSA26-ZtTA; Stock No. 012266), the resulting double mutant mice allow tdTomato and Syp-GFP expression to be determined by the tissue-specific Cre recombinase expression of a third strain of the researchers choosing. For example, when TRE-Bi-SG-T transgenic mice are bred with floxed-STOP-tTA mice (ROSA26-ZtTA; Stock No. 012266) and a neural-specific Cre recombinase strain (for example: Foxg1-Cre; Stock Nos. 004337 or 006084), the resulting triple mutant mice should exhibit labeling of whole neurons in red and presynaptic terminals in green in the absence of dox."
  2. Related Disease
    cardiovascular system disease
  3. Related Disease
    disease of cellular proliferation
  4. Website(s)
    http://jaxmice.jax.org/strain/012345.html
  5. Biological process studied
    Reproduction
  6. Biological process studied
    Developmental process
  7. Biological process studied
    neurological system process
  8. Genetic Alteration(s)
    Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo
  9. Location
    Steven Thomas Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-11-02T16:13:42.694-04:00
  4. creator
    apernot (Anne Claire Pernot)
  5. modified
    2013-11-08T11:22:16.381-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016