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B6.Cg-Akt2(tm1.1Mbb/J)

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000144-dadf-e443-3e6a-4e4480000000

Resource Type

  1. Mus musculus

Properties

  1. Exchange facilitator
    http://jaxmice.jax.org/strain/006966.html
  2. Resource Description
    "Mice were generated with a targeted disruption in the Akt2 locus by homologous recombination. The targeting vector was designed to insert LoxP sites flanking the sequence containing the coding exons 4 and 5 Mice harboring the targeted allele were identified by Southern blotting and were mated to transgenic mice expressing Cre recombinase. The progeny carrying both the Cre transgene and the targeted allele were mated with wild-type (WT) mice to obtain offspring in which the Cre transgene was segregated away and the targeted allele was excised, as determined by the polymerase chain reaction (PCR) and Southern blotting, respectively. These mice were mated inter se to produce offspring with homozygous deletions of Akt2."
  3. Additional Name
    AKT2 deficient
  4. Additional Name
    Akt2 KO
  5. Additional Name
    Akt2−/−
  6. Related Publication or Documentation
    Akt1 and Akt2 are required for alphabeta thymocyte survival and differentiation
  7. Related Publication or Documentation
    AKT1 and AKT2 maintain hematopoietic stem cell function by regulating reactive oxygen species
  8. Related Publication or Documentation
    Natural and inducible TH17 cells are regulated differently by Akt and mTOR pathways
  9. Website(s)
    http://www.informatics.jax.org/allele/key/5945
  10. Website(s)
    http://www.sciencemag.org/content/292/5522/1728.full
  11. Parental Strain Name
    C57BL/6
  12. Biological process studied
    Immune system process
  13. Biological process studied
    hematopoietic stem cell differentiation
  14. Genetic Alteration(s)
    Akt2[tm1.1Mbb]
  15. Developed by
    Birnbaum Laboratory
  16. Phenotype Findings
    Insulin Level
  17. Location
    Koretzky Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2014-03-19T11:33:43.271-04:00
  4. creator
    laavanya (Laavanya Sankaranarayanan)
  5. modified
    2014-03-25T10:49:28.090-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016