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Perkin Elmer Operetta

eagle-i ID


Resource Type

  1. Fluorescence microscope


  1. Resource Description
    "The Perkin Elmer Operetta is an inverted epifluoresence microscope that utilizes a laser auto-focus option to automate acquisition of high-content images in up to 4 channels (three fluorescent plus brightfield) from 96- and 384-well microtiter plates or slides. It is equipped with a xenon arc lamp excitation source, a motorized objective wheel with bar-coded, interchangeable objectives (2X, 4X, 10X, 20X, 40X), an 8 position excitation filter wheel with matched dichroics, an 8 position emission filter wheel with interchangeable, bar coded filters for maximum detection of fluorescent labels, and a 1.3 megapixel, 14-bit Peltier cooled CCD camera. The Operetta uses Harmony, which includes a seamlessly integrated database for management of images, experimental information, data analysis, and metadata information. This provides the capability for convenient queries using a sortable data tree and cellular image analysis software to select a standard image analysis routine or develop custom analysis sequences to fit a project’s specific needs. Data can be stored locally on a 3.0 TB drive for short periods of time. An additional 10.0 TB network accessible RAID server pre-configured to immediately handle storage, management, and analysis of high-content data using the Columbus web-based interface is available in cooperation with the Bioinformatics Resource and the Center for Systems and Computational Biology. Columbus provides database access to an unlimited number of authorized users, which enables them to use Acapella and develop custom analysis sequences that cannot be achieved using standard analysis algorithms."
  2. Manufacturer
    PerkinElmer Inc.
  3. Website(s)
  4. Location
    Molecular Screening and Protein Expression Facility (Wistar)
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016