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Biacore T200

eagle-i ID


Resource Type

  1. Surface plasmon resonance instrument


  1. Resource Description
    "The Biacore T200 (GE Healthcare) utilizes the Surface Plasmon Resonance (SPR) technology to study biomolecular binding events on a chip surface. This technology has the advantage of being able to work with biomolecules in their native forms, without the need of introduction of label or tag (referred to ‘label-free’). The basic principle involves immobilization of a ligand on a sensor chip followed by continuous delivery of an analyte by a microfluidic system. The SPR-based technology continuously monitors the changes of mass deposition on the detector surface, and allows sensitive and reliable characterization of biomolecular interactions. Any protein, DNA, RNA, lipid, carbohydrate, polysaccharide, cell, virus, drug, drug like molecule (organic or inorganic) can be used as the ligand or analyte. Since the detection system is based on measuring total mass on the sensor chip surface neither ligand nor analyte has to be tagged. The SPR technology can provide quantitative data on:    • Specificity: How specific is the binding between two molecules?    • Concentration: How much of a given molecule is present and active?    • Kinetics: What is the rate of association and dissociation?    • Affinity: How strong is the binding?"
  2. Manufacturer
    General Electric Company
  3. Website(s)
  4. Location
    Molecular Screening and Protein Expression Facility (Wistar)
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016