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The RNAi Consortium (TRC) Mm 1.0 shRNA library

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000148-8f8d-61ea-6c8d-188780000000

Resource Type

  1. shRNA construct library

Properties

  1. Resource Description
    "The Wistar Institute has purchased bacterial glycerol stocks of the complete (human and mouse) TRC shRNA library (Moffat, J. et al Cell, (2006) 124:1283-1298). The human library (TRC-Hs1.0) targets 15,000 annotated human genes and consists of 80,700 precloned constructs. The mouse library (TRC-Mm1.0) targets 15,000 annotated mouse genes and consists of 76,800 precloned constructs. The hairpin sequences, a 21-base stem and a 6-base loop, are each cloned into the pLKO.1 vector, which allows production of replication-incompetent lentiviral particles, transient or stable expression of the shRNA, and antibiotic (puromycin) selection of transfected or infected cells. On average, each gene is targeted by ~5 constructs. The shRNA sequences for a given target are distributed throughout the cDNA sequence of a target, including a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs."
  2. Additional Name
    Mouse short hairpin RNA library
  3. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/molecular-screening-facility/shrna-resource
  4. Library Count
    76800
  5. Location
    Molecular Screening Facility (Wistar)
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2014-09-19T16:15:21.484-04:00
  4. creator
    fcoldren
  5. modified
    2014-09-19T16:25:15.221-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016