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Whole genome pooled library distribution

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000148-8f9a-f7c0-6c8d-188780000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The Resource has prepared pools of plasmid DNA for clones from the TRC shRNA library by species and produced concentrated viral vector (³5x108 TU/ml) for infection of mammalian cell cultures. Each plasmid DNA/viral vector pool consists of ~9600 constructs with the whole genome of each species (human and mouse) covered by 12 pools, respectively. The pools are not organized by GO annotated gene families. Each viral shRNA vector pool and control vector (e.g. empty, non-targeting shRNA) will be provided as 2 x 10ul frozen aliquots in PBS that have been verified to be at least 5 x 108 TU/ml, as determined using a p24 assay."
  3. Related Resource
    The RNAi Consortium (TRC) Hs1.0 shRNA library
  4. Related Resource
    The RNAi Consortium (TRC) Mm 1.0 shRNA library
  5. Access Restriction(s)
    Clones and/or virus may not be distributed to scientists outside of the Wistar Institute.
  6. Service Provided by
    Molecular Screening Facility (Wistar)
  7. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/molecular-screening-facility/shrna-resource
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2014-09-19T16:30:40.133-04:00
  4. creator
    fcoldren
  5. modified
    2014-09-19T16:32:48.004-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016