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Fluidigm SNPtype SNP genotyping assay

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000149-43f1-b6cf-ed60-4b1280000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Recent advances in nanofluidics technology have made possible the use of integrated fluidic circuits (IFCs) for high-throughput real-time PCR. Nanoliter-scale quantities of samples and reagents are channeled into thousands of nanoliter-scale chambers in which distinct real-time PCRs can be run. Fluidigm Dynamic Array integrated fluidic circuits (IFCs) deliver a new level of efficiency and throughput for real time PCR and genotyping that is compatible with existing TaqMan genotyping assays. Potentially, this technology allows up to 9216 individual TaqMan reactions to be run in a single experiment, with the promise of considerable reagent and time savings achievable from using nanofluidics arrays, compared with standard TaqMan genotyping on ABI PRISM 7900HT. Dynamic Array IFCs have an on-chip network of microfluidic channels, chambers, and valves that automatically assemble individual PCR reactions, decreasing the number of pipetting steps required by up to 100 fold. The available chip formats that can be used in our facility are: • 48.48 - 48 samples x 48 assays (single use) • 96.96 - 96 samples x 96 assays (single use) Fluidigm SNPtype Assays can be used instead of Taqman assays that provide a custom, high- throughput, low-cost single nucleotide polymorphism (SNP) genotyping solution which enables rapid assay design and polymorphism screening. SNPtype Assays employ tagged, allele-specific PCR primers and a common reverse primer. A universal probe set is used in every reaction, producing uniform fluorescence while significantly reducing both startup and running costs. The assays provide excellent results on Dynamic Array IFCs and are compatible with existing workflows."
  3. Service Fee URL
    https://pathbio.med.upenn.edu/pbr/portal/mpf/fees.php
  4. Related Resource
    Fluidigm BioMark HD
  5. Service Provided by
    Molecular Profiling Facility (Penn)
  6. Website(s)
    https://pathbio.med.upenn.edu/pbr/portal/mpf/technology.php#singleassayabiprism
  7. Related Technique
    SNP genotyping assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2014-10-24T16:56:37.669-04:00
  4. creator
    fcoldren
  5. modified
    2016-04-18T14:28:41.723-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016