eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Taqman gene expression assay

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000149-533f-4a7e-ed60-4b1280000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    Services include: <strong>cDNA synthesis</strong> <strong>Gene Expression Assay by Taqman</strong> "Real-time detection of PCR products is made possible by including in the reaction a fluorescent molecule that reports an increase in the amount of DNA with a proportional increase in fluorescent signal. The fluorescent chemistries employed for this purpose include DNA-binding dyes and fluorescently labeled sequence specific primers or probes. Specialized thermal cyclers equipped with fluorescence detection modules are used to monitor the fluorescence as amplification occurs. The measured fluorescence reflects the amount of amplified product in each cycle. Key Features and Benefits of the Assay • One or Two step RT-PCR for RNA quantitation • Plus/Minus assays using an internal positive control (IPC)"
  3. Service Fee URL
    https://pathbio.med.upenn.edu/pbr/portal/mpf/fees.php
  4. Related Resource
    QuantStudio 12K Flex Real-Time PCR System
  5. Service Provided by
    Molecular Profiling Facility (Penn)
  6. Website(s)
    https://pathbio.med.upenn.edu/pbr/portal/mpf/technology.php#singleassayabiprism2
  7. Related Technique
    Gene expression assay
  8. Related Technique
    Real-time PCR
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2014-10-27T16:16:14.235-04:00
  4. creator
    fcoldren
  5. modified
    2016-04-18T14:44:30.598-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016