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Digital PCR service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000149-5359-c22d-ed60-4b1280000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    Services include: <strong>12.765 Digital Array 48.770 Digital Array</strong> "Digital PCR works by partitioning a sample into many individual real-time PCR reactions; some portion of these reactions contain the target molecule (positive) while others do not (negative). Following PCR analysis, the fraction of negative reactions is used to generate an absolute count of the number of target molecules in the sample, without reference to standards or endogenous controls." "To meet the need for absolute quantitation and detection of target sequences Fluidigm qdPCR 37K™ IFC has the ability to perform four-plex detection. Since other platforms are limited to two-plex capabilities up to 80% savings per sample is expected when an experiment demands higher-plex capabilities, without sacrificing accuracy. Key Features and Benefits of the Assay • Rare Allele Detection • Low-Fold Copy Number Discrimination • Absolute Quantification of Viral Load • Absolute Quantification of Nucleic Acid Standards • Absolute Quantification of Next-Generation Sequencing Libraries"
  3. Service Fee URL
    https://pathbio.med.upenn.edu/pbr/portal/mpf/fees.php
  4. Related Resource
    Fluidigm BioMark HD
  5. Service Provided by
    Molecular Profiling Facility (Penn)
  6. Website(s)
    https://pathbio.med.upenn.edu/pbr/portal/mpf/technology.php#gigitalpcr1
  7. Related Technique
    Multiplex bead assay
  8. Related Technique
    Real-time PCR
  9. Related Technique
    Copy number variation profiling
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow owner
    fcoldren
  2. workflow state
    Published
  3. contributor
    fcoldren
  4. created
    2014-10-27T16:45:16.235-04:00
  5. creator
    fcoldren
  6. modified
    2016-04-18T14:36:18.120-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016