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Single-Molecule Optical Trap, Sub-Pixel Tracking and Polarization Microscopes

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Resource Type

  1. Total internal reflection fluorescence instrument


  1. Resource Description
    The microscopes were designed and built by the laboratory of Dr. Yale E. Goldman using modified Nikon TE-2000 and TE-I microscopes and Photometrics Cascade II and Evolve EMCCD cameras. Prism-type and objective-type total internal reflection fluorescence microscopes equipped with optics on the excitation and emission pathways for detecting changes in orientation and rotational mobility of single fluorescently molecules and for applying pN-level mechanical forces. The instruments are capable of tracking the 3-dimensional position of fluorescent molecules with nanometer-level precision in vitro and in live cells. Excitation and emission wavelengths are optimized for macromolecules fluorescently-labeled with probes in the visible wavelength range.
  2. Contact
    Goldman, Yale E., MD, PhD
  3. Access Restriction(s)
    These instruments are available via collaboration with the laboratory of Dr. Yale E. Goldman.
  4. Related Publication or Documentation
    Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy
  5. Related Publication or Documentation
    The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V
  6. Related Publication or Documentation
    Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors
  7. Related Publication or Documentation
    Parallax: High Accuracy Three-Dimensional Single Molecule Tracking Using Split Images
  8. Developed by
    Goldman Laboratory
  9. Location
    Advanced Optical and Force Microscopy Facility (Penn)
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016