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Construction of a targeting vector

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-4b28-b523-d034-1ff680000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The final price of constructing the targeting vector in the Core will depend on the number and nature of cloning steps. This is usually in the range of $3,000 - $6,000. BAC recombineering is used for construction in the majority of cases to ensure long arms of homology. If outside vendors are used for construction, vendors charges will apply. The decision whether to use outside vendors or the UPenn Core is taken jointly by the client’s lab and PGT after thorough discussion of the technical specifics and optimal timing of the project. In some cases synthesis of up to 3000 nt DNA fragments may be required. PGT has extensive experience using synthetic DNA for vector construction. PGT has extensive experience in the construction of all types of targeting vectors, including those introducing knockin mutations, reporter cassettes, stop cassettes and floxed alleles. In addition, vectors targeting tissue and/or drug-inducible transgenes to specific loci, including the Rosa 26 locus, can be constructed."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/pgt/services.shtml
  4. Service Provided by
    Penn Gene Targeting Core and Laboratory (Penn)
  5. Related Publication or Documentation
    Cutting edge: Conditional MHC class II expression reveals a limited role for B cell antigen presentation in primary and secondary CD4 T cell responses
  6. Related Publication or Documentation
    CHD5 is required for spermiogenesis and chromatin condensation
  7. Related Publication or Documentation
    An Acvr1 R206H knock-in mouse has fibrodysplasia ossificans progressiva
  8. Website(s)
    http://www.med.upenn.edu/genetics/pgt/services.shtml
  9. Related Technique
    Construct engineering
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-02T11:40:45.115-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-11T14:57:51.052-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016