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Identification of homologous recombinant ES clones using Southern and PCR

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-4b2c-773b-d034-1ff680000000

Resource Type

  1. Analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Design and testing of suitable Southern blot probes to detect correct insertion of the targeting vector along its entire length and at least one genomic PCR primer pair to detect the presence of a targeted mutation: Flat price of $1,700 if only one Southern probe and one genomic PCR primer pair are is needed. $2,700 if two working Southern probes (binding just outside of opposite sides of the integrated vector) and one genomic PCR primer pair are required."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/pgt/services.shtml
  4. Service Provided by
    Penn Gene Targeting Core and Laboratory (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/pgt/services.shtml
  6. Related Technique
    Polymerase chain reaction
  7. Related Technique
    Southern blot analysis
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-02T11:43:43.202-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-11T15:01:08.966-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016