Southern screening, including isolation of genomic DNA

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "For 40 ES clones: $1,600. Without isolation of genomic DNA: $1,000. Usually we obtain between 5 and 30 initial positive clones. For certain targeted genes targeting efficiency is naturally low due to epigenetic / structural issues at the targeted locus. If an electroporation must be repeated for this reason we will charge only 70% of the original price for the electroporation."

3. Service Fee URL

   http://www.med.upenn.edu/genetics/pgt/services.shtml

4. Service Provided by

   Penn Gene Targeting Core and Laboratory (Penn)

5. Website(s)

   http://www.med.upenn.edu/genetics/pgt/services.shtml

6. Related Technique

   DNA extraction

7. Related Technique

   Southern blot analysis