IFNγ ELISPOT (mouse and NHP) service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The ELISPOT detecting interferon (IFNγ) has become widely used as a means of quantization of T cell-mediated immune responses (CD4+ or CD8+) and is becoming the assay of choice for clinical trials for detecting T cell responses to gene therapy product and candidate vaccines. It is relatively rapid; sensitive, and allows the screening of multiple samples on a single day. The ELISPOT assay is based on the principle of the ELISA detecting antigen-induced secretion of cytokines trapped by an immobilized antibody and visualized by an enzyme-coupled second antibody. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image002_001.gif" alt="figure from core website"> Figure 1 : IFNg ELISPOT of mice splenocytes. Host T cell responses to AAV2, 7, or 8 capsid studied in C57BL/6 mice injected i.m. with 1x1011 GC of rAAV2, 2/7, or 2/8 vectors."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Additional Name

   Interferon-gamma ELISPOT

5. Topic

   Mus musculus

6. Topic

   Immune response

7. Service Provided by

   Immunology Core- Gene Therapy Program

8. Website(s)

   http://www.med.upenn.edu/gtp/immunology_tcells.shtml

9. Related Technique

   ELISPOT assay