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IFNγ ELISPOT (mouse and NHP) service

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "The ELISPOT detecting interferon (IFNγ) has become widely used as a means of quantization of T cell-mediated immune responses (CD4+ or CD8+) and is becoming the assay of choice for clinical trials for detecting T cell responses to gene therapy product and candidate vaccines. It is relatively rapid; sensitive, and allows the screening of multiple samples on a single day. The ELISPOT assay is based on the principle of the ELISA detecting antigen-induced secretion of cytokines trapped by an immobilized antibody and visualized by an enzyme-coupled second antibody. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image002_001.gif" alt="figure from core website"> Figure 1 : IFNg ELISPOT of mice splenocytes. Host T cell responses to AAV2, 7, or 8 capsid studied in C57BL/6 mice injected i.m. with 1x1011 GC of rAAV2, 2/7, or 2/8 vectors."
  3. Service Fee URL
  4. Additional Name
    Interferon-gamma ELISPOT
  5. Topic
    Mus musculus
  6. Topic
    Immune response
  7. Service Provided by
    Immunology Core- Gene Therapy Program
  8. Website(s)
  9. Related Technique
    ELISPOT assay
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016