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IFNγ ELISPOT (mouse and NHP) service
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000014b-7f4b-8a40-d034-1ff680000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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"The ELISPOT detecting interferon (IFNγ) has become widely used as a means of quantization of T cell-mediated immune responses (CD4+ or CD8+) and is becoming the assay of choice for clinical trials for detecting T cell responses to gene therapy product and candidate vaccines. It is relatively rapid; sensitive, and allows the screening of multiple samples on a single day. The ELISPOT assay is based on the principle of the ELISA detecting antigen-induced secretion of cytokines trapped by an immobilized antibody and visualized by an enzyme-coupled second antibody.
<img src="http://www.med.upenn.edu/gtp/user_images/clip_image002_001.gif" alt="figure from core website">
Figure 1 : IFNg ELISPOT of mice splenocytes. Host T cell responses to AAV2, 7, or 8 capsid studied in C57BL/6 mice injected i.m. with 1x1011 GC of rAAV2, 2/7, or 2/8 vectors."
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Service Fee URL
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https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
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Additional Name
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Interferon-gamma ELISPOT
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Topic
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Mus musculus
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Topic
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Immune response
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Service Provided by
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Immunology Core- Gene Therapy Program
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Website(s)
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http://www.med.upenn.edu/gtp/immunology_tcells.shtml
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Related Technique
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ELISPOT assay
