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IFNγ ELISPOT (mouse and NHP) service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-7f4b-8a40-d034-1ff680000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The ELISPOT detecting interferon (IFNγ) has become widely used as a means of quantization of T cell-mediated immune responses (CD4+ or CD8+) and is becoming the assay of choice for clinical trials for detecting T cell responses to gene therapy product and candidate vaccines. It is relatively rapid; sensitive, and allows the screening of multiple samples on a single day. The ELISPOT assay is based on the principle of the ELISA detecting antigen-induced secretion of cytokines trapped by an immobilized antibody and visualized by an enzyme-coupled second antibody. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image002_001.gif" alt="figure from core website"> Figure 1 : IFNg ELISPOT of mice splenocytes. Host T cell responses to AAV2, 7, or 8 capsid studied in C57BL/6 mice injected i.m. with 1x1011 GC of rAAV2, 2/7, or 2/8 vectors."
  3. Service Fee URL
    https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
  4. Additional Name
    Interferon-gamma ELISPOT
  5. Topic
    Mus musculus
  6. Topic
    Immune response
  7. Service Provided by
    Immunology Core (Penn)
  8. Website(s)
    http://www.med.upenn.edu/gtp/immunology_tcells.shtml
  9. Related Technique
    ELISPOT assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-12T14:45:02.165-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-12T15:43:30.374-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016