Validated human IFNγ ELISPOT for Clinical Trials

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "A properly conducted trial requires the assay to be validated. Validation is ensures that an assay is: - Specific: measures in a proven way what it is supposed to measure - Sensitive: has determined upper and lower limits of quantitation - Precise: has determined maximum intra- and inter assay variations - Robust: is independent of the operator and the laboratory in which is carried out For our validation assay we used human PBMC samples from The University of Pennsylvania Hospital. As stimulants we used CEF (MHC class I restricted T- cell epitopes from CM, Epstein Barr and influenza virus), SEB (Staphylococcal enterotoxin B) and PMA+ION (calcium ionophore) An automated ELISPOT reader (AID) was used to minimize the operator dependent influence on the quantitative output."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Additional Name

   Validated human interferon-gamma ELISPOT for Clinical Trials

5. Topic

   Homo sapiens

6. Topic

   Immune response

7. Service Provided by

   Immunology Core- Gene Therapy Program

8. Website(s)

   http://www.med.upenn.edu/gtp/immunology_tcells.shtml

9. Related Technique

   Clinical trial technique

10. Related Technique

    ELISPOT assay