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Validated human IFNγ ELISPOT for Clinical Trials

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Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "A properly conducted trial requires the assay to be validated. Validation is ensures that an assay is: - Specific: measures in a proven way what it is supposed to measure - Sensitive: has determined upper and lower limits of quantitation - Precise: has determined maximum intra- and inter assay variations - Robust: is independent of the operator and the laboratory in which is carried out For our validation assay we used human PBMC samples from The University of Pennsylvania Hospital. As stimulants we used CEF (MHC class I restricted T- cell epitopes from CM, Epstein Barr and influenza virus), SEB (Staphylococcal enterotoxin B) and PMA+ION (calcium ionophore) An automated ELISPOT reader (AID) was used to minimize the operator dependent influence on the quantitative output."
  3. Service Fee URL
  4. Additional Name
    Validated human interferon-gamma ELISPOT for Clinical Trials
  5. Topic
    Homo sapiens
  6. Topic
    Immune response
  7. Service Provided by
    Immunology Core- Gene Therapy Program
  8. Website(s)
  9. Related Technique
    Clinical trial technique
  10. Related Technique
    ELISPOT assay
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016