See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.
Validated human IFNγ ELISPOT for Clinical Trials
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000014b-7f57-e92f-d034-1ff680000000
Resource Type
Properties
-
-
Fee for service
-
Yes
-
-
Resource Description
-
"A properly conducted trial requires the assay to be validated. Validation is ensures that an assay is:
- Specific: measures in a proven way what it is supposed to measure
- Sensitive: has determined upper and lower limits of quantitation
- Precise: has determined maximum intra- and inter assay variations
- Robust: is independent of the operator and the laboratory in which is carried out
For our validation assay we used human PBMC samples from The University of Pennsylvania Hospital. As stimulants we used CEF (MHC class I restricted T- cell epitopes from CM, Epstein Barr and influenza virus), SEB (Staphylococcal enterotoxin B) and PMA+ION (calcium ionophore)
An automated ELISPOT reader (AID) was used to minimize the operator dependent influence on the quantitative output."
-
-
Service Fee URL
-
https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
-
-
Additional Name
-
Validated human interferon-gamma ELISPOT for Clinical Trials
-
-
Topic
-
Homo sapiens
-
-
Topic
-
Immune response
-
-
Service Provided by
-
Immunology Core- Gene Therapy Program
-
-
Website(s)
-
http://www.med.upenn.edu/gtp/immunology_tcells.shtml
-
-
Related Technique
-
Clinical trial technique
-
-
Related Technique
-
ELISPOT assay
