Cultured ELISPOT and ICCS

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The IFNg ELISPOT after “in vitro” expansion in the presence of IL2 and IL7 increases the sensitivity of the assay allowing us to detect even a small central memory-mediated T-cell response. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image008.gif" alt="Figure 4 from core website"> Figure 4 : Cultured ELISPOT to detect central memory capsid T cells in human. PBMCs from human were expanded in vitro prior to assay as follows. Freshly isolated cells are cultured for 7 days in medium with peptides pools in the presence of hIL2 and hIL-7 at 2x106 cells/ml. Cells are harvested and analyzed by ELISPOT measuring production of IFN-γ. The data are presented for two samples, one that showed high frequencies in most peptide pools and one in which the frequencies were intermediate. Indicated are the peptide pools for the different serotypes 2, 7, 8 and 9. SEB and PMA are positive controls."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Topic

   Immune response

5. Service Provided by

   Immunology Core- Gene Therapy Program

6. Website(s)

   http://www.med.upenn.edu/gtp/immunology_tcells.shtml

7. Related Technique

   ELISPOT assay