eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Lymphoproliferation assay (CFSE) service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-7f65-be71-d034-1ff680000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "A key feature of the adaptive immune response is the ability of clones of antigen-specific lymphocytes to rapidly proliferate and differentiate into effector cells. The intracellular fluorescent dye, CFSE, has been found to be particularly effective at monitoring lymphocyte division in many experimental situations. The tracking of lymphocyte division using CFSE has become a routine procedure in many laboratories. In particular, the procedure has been used for the analysis of cell division in vivo in mice and in vitro using mouse and human lymphocytes. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image010.gif" alt="Figure 5 on core website"> Figure 5 : PBMCs isolated from a Cynomolgus macaque were stained with CFSE and stimulated with PHA for 3 days. PBMCs were also stained for CD4 and CD8."
  3. Service Fee URL
    https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
  4. Service Provided by
    Immunology Core (Penn)
  5. Website(s)
    http://www.med.upenn.edu/gtp/immunology_tcells.shtml
  6. Related Technique
    Cell proliferation assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-12T15:09:02.737-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-12T15:43:35.772-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016