Lymphoproliferation assay (CFSE) service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "A key feature of the adaptive immune response is the ability of clones of antigen-specific lymphocytes to rapidly proliferate and differentiate into effector cells. The intracellular fluorescent dye, CFSE, has been found to be particularly effective at monitoring lymphocyte division in many experimental situations. The tracking of lymphocyte division using CFSE has become a routine procedure in many laboratories. In particular, the procedure has been used for the analysis of cell division in vivo in mice and in vitro using mouse and human lymphocytes. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image010.gif" alt="Figure 5 on core website"> Figure 5 : PBMCs isolated from a Cynomolgus macaque were stained with CFSE and stimulated with PHA for 3 days. PBMCs were also stained for CD4 and CD8."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Service Provided by

   Immunology Core- Gene Therapy Program

5. Website(s)

   http://www.med.upenn.edu/gtp/immunology_tcells.shtml

6. Related Technique

   Cell proliferation assay