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In vivo CTL assay (mouse) service
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000014b-7f69-410b-d034-1ff680000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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"This assay allows the detection of cytolytic activity specific for a peptide epitope in-vivo in mice. One group of target cells is labeled with a higher concentration of CFSE dye and pulsed with the antigenic peptide epitope. While the other group of target cells is labeled with a lower concentration of CFSE and pulsed with an irrelevant peptide. They are mixed in a 1:1 ratio and injected i.v. into immunized and naïve mice. A deviation from the 1:1 ratio when the mice are sampled indicates specific lysis of the cells with the relevant epitope on the surface. Normal spleen cells are used as target cells.
<img src="http://www.med.upenn.edu/gtp/user_images/clip_image012.gif" alt="Figure 6 from core website">
Figure 6: In-vivo CTL assay in Balb/c mice immunized against Hiv-1 gag. Balb/c mice were immunized IM with AdH5-Gag. On Day 35, two populations of naïve target cells were labeled with CFSE followed by pulsing with relevant Gag and irrelevant RT epitopes respectively. Target cells were introduced in a 1:1 ratio into the immunized mice and naïve control mice. Eighteen hrs post transfer, splenocytes were harvested and subjected to flow cytometry to measure the relative proportion of CFSE hi-vs CFSE lo cells."
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Service Fee URL
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https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
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Additional Name
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Cytotoxic T lymphocyte assay
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Topic
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Mus musculus
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Topic
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Immune response
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Service Provided by
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Immunology Core- Gene Therapy Program
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Website(s)
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http://www.med.upenn.edu/gtp/immunology_tcells.shtml
