In vivo CTL assay (mouse) service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "This assay allows the detection of cytolytic activity specific for a peptide epitope in-vivo in mice. One group of target cells is labeled with a higher concentration of CFSE dye and pulsed with the antigenic peptide epitope. While the other group of target cells is labeled with a lower concentration of CFSE and pulsed with an irrelevant peptide. They are mixed in a 1:1 ratio and injected i.v. into immunized and naïve mice. A deviation from the 1:1 ratio when the mice are sampled indicates specific lysis of the cells with the relevant epitope on the surface. Normal spleen cells are used as target cells. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image012.gif" alt="Figure 6 from core website"> Figure 6: In-vivo CTL assay in Balb/c mice immunized against Hiv-1 gag. Balb/c mice were immunized IM with AdH5-Gag. On Day 35, two populations of naïve target cells were labeled with CFSE followed by pulsing with relevant Gag and irrelevant RT epitopes respectively. Target cells were introduced in a 1:1 ratio into the immunized mice and naïve control mice. Eighteen hrs post transfer, splenocytes were harvested and subjected to flow cytometry to measure the relative proportion of CFSE hi-vs CFSE lo cells."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Additional Name

   Cytotoxic T lymphocyte assay

5. Topic

   Mus musculus

6. Topic

   Immune response

7. Service Provided by

   Immunology Core- Gene Therapy Program

8. Website(s)

   http://www.med.upenn.edu/gtp/immunology_tcells.shtml