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Transduction inhibition based neutralizing antibody assay (AAV and Adv vectors)

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-7f7a-6497-d034-1ff680000000

Resource Type

  1. Analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    <img src="http://www.med.upenn.edu/gtp/user_images/ig4.gif" alt="top image on core website"> "<strong>Neutralizing antibodies</strong> Principle: Virus neutralization assays are performed to assess the potential of antibody to block virus transduction of susceptible cell lines. The ability of antibodies to block adenovirus infection of Hela cells or AAV2 infection of 84-31 (293 cells stably expressing Ad-E4) in vitro is analyzed utilizing Green Fluorescent Protein as a reporter*. For the assay, various dilutions of antibodies pre-incubated with moi of 100 for adenovirus and 1000 for AAV reporter viruses for 1 hour at 37°C, is added to 90% confluent cell cultures. Cells are incubated for 20 hours. Expression of GFP is quantitated by manually counting under a UV-microscope and LacZ by an automated luminometer. The neutralizing titer of antibody is calculated by the highest dilution of the sera where 50% of the cells are green. *or LacZ"
  3. Service Fee URL
    https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php
  4. Service Provided by
    Immunology Core (Penn)
  5. Website(s)
    http://www.med.upenn.edu/gtp/immunology_bcells.shtml
  6. Related Technique
    Virus neutralization
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-12T15:30:09.385-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-12T15:43:36.746-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016