Infection inhibition based neutralizing antibody assay (Adv wt virus)

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The principle of this assay is the same as the transduction inhibition neutralizing antibody assay but in the infection inhibition assay we use wild type virus and A549 cells. Infection is quantified by immunostaining using a goat anti-hexon antibody raised against a conserve region present in all identified Adeno types. <img src="http://www.med.upenn.edu/gtp/user_images/clip_image002.gif" alt="bottom figure on core website"> Figure : Infection inhibition neutralizing antibody assay to HAdv5 and SAdV24 using polyclonal rabbit antisera to HAdv5, SAdV23, SAdV24 and Human pooled IgG. Neutralization titers are highlighted."

3. Service Fee URL

   https://somapps.med.upenn.edu/pbr/portal/gimm/fees.php

4. Service Provided by

   Immunology Core- Gene Therapy Program

5. Website(s)

   http://www.med.upenn.edu/gtp/immunology_bcells.shtml