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iPSC derivation from blood using lentiviral vector or Sendai virus

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000014b-a2ec-c8b0-d034-1ff680000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "A minimum of 8 mls of blood is required for this service. Blood should be collected in CPT tube (BD biosciences) or other heparinized or EDTA tubes and delivered fresh to the Core. Please contact the Core director if you are submitting peripheral blood mononuclear cells (PBMCs). This service takes 3-6 months to complete and includes the following: • Isolation and cryopreservation of PBMCs from blood • Expansion of erythroblasts from PBMCs and mycoplasma testing • Transduction of erythroblasts with lentiviral vector or Sendai viral vectors • iPSC colony picking • Expansion of 4 iPSC clones • Mycoplasma testing • FACS analysis of 2 pluripotency markers (TRA-1-60 and SSEA4) • Cryopreservation of iPSC lines (4-6 vials/clone) • Will deliver 4 clones (frozen or live culture) at passage >5 Notes for iPSC reprogramming services: Inherent to iPSC reprogramming, a certain percentage of cells will fail to produce iPS cells that is likely cell specific and not due to technical reasons. If we do not obtain stable colonies after the first round of reprogramming, we will repeat the process. However, if the second round fails, we will not repeat again and will only charge 50% of the service fee."
  3. Service Provided by
    Induced Pluripotent Stem Cell Core Facility (Penn)
  4. Website(s)
    http://www.med.upenn.edu/ipsccore/services.shtml
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2015-02-19T12:41:18.066-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-19T17:22:24.370-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016