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High throughput sequencing service (Collaboration)

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "Customers can collaborate with Microbiome Center staff for help with designing and analyzing microbiome-related projects. In the second format, customers will collaborate with the center to design and carry out complete projects. In this work flow, customers will begin by meeting with Center staff. Initial consultation on experimental design will be free of charge, subsequent use of Center Resources will be charged according to our cost schedule. The analysis will be fully planned, including sample size assessment and construction of the statistical framework for analysis. Customers will be provided with a cost estimate. Customers will then prepare complete metadata tables for the analysis, and present written documentation of IRB approval. Samples are then sent to the Center, DNA is purified, sequencing libraries are prepared, and sequence is determined. The pre-specified analysis is then carried out, and reported to customers. Any unused sample is also returned to customers. Additional analysis is available on a fee for service basis." "The core has three work-flows for sequence acquisition: 1. High throughput shotgun metagenomic analysis of large microbiome sample sets. Sequencing will take place on the two HiSeq 2500 instruments. 2. High throughput analysis of amplicons, such as 16S rRNA gene tags to characterize bacterial community membership. Sequencing will take place on the MiSeq instrument. 3. Complete characterization of bacterial genomes. Sequencing will take place on the PacBio RS II instrument. Click here to get <a href="http://microbiome.research.chop.edu/images/pdfs/guideline04.pdf">general sample submission guidelines</a> and specific <a href="http://microbiome.research.chop.edu/images/pdfs/PacbioGuidelines.pdf">PacBio instructions</a>."
  3. Related Resource
    Illumina HiSeq 2500
  4. Related Resource
    Illumina MiSeq
  5. Related Resource
    Pacific Biosciences RS II
  6. Access Restriction(s)
  7. Service Provided by
    CHOP Microbiome Center (Sequencing and Analytical Resource) (CHOP)
  8. Website(s)
  9. Related Technique
    Metagenomics analysis
  10. Related Technique
    Data analysis
  11. Related Technique
    RNA sequencing
  12. Related Technique
    Whole genome sequencing
  13. Related Technique
    DNA sequencing assay
  14. Related Technique
    Nucleic acid library preparation
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    ggrant (Gregory Grant)
  3. created
  4. creator
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016