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Transfection of mammalian cells with or without selection of stable cell lines

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000154-3f06-6a46-8aef-b65680000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The CCS will transfect suspension 293 cells (or another cell line as requested). We will then scale up the cells for protein production and provide the researcher with supernatant and/or cell pellet. Upon request we are able to purify antibodies or tagged recombinant proteins. For selection of stable cell lines we will select with G418 and pick up to 24 positive clones. Expression of the specific recombinant protein is verified by Western blot with an antibody that is to be provided by the researcher. Please call us with inquiries about customized orders."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/cellctr/services/transmamcells.shtml
  4. Service Provided by
    Cell Center Services Facility (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/cellctr/services/transmamcells.shtml
  6. Related Technique
    Transfection
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2016-04-22T13:32:08.592-04:00
  4. creator
    fcoldren
  5. modified
    2016-04-22T13:32:16.570-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016