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PCR, PCR Clean-Up & Cloning service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000154-4087-097b-8aef-b65680000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "PCR of genomic DNA or BAC DNA are done using custom primers. Primers can be designed at the facility. The optimization and the clean up of PCR products are done using various procedures including exo-sap, column and gel purification. The cleaned up products can be directly processed for Sanger sequencing at the facility or used for cloning. PCR products are cloned using Invitrogen's TA cloning system."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/dnaseq/pricing.shtml#molbio
  4. Service Provided by
    DNA Sequencing Facility (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/dnaseq/pcr.shtml
  6. Related Technique
    Polymerase chain reaction
  7. Related Technique
    Recombinant plasmid cloning
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2016-04-22T20:32:56.649-04:00
  4. creator
    fcoldren
  5. modified
    2016-04-22T20:33:07.858-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016