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Cloning and subcloning service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000154-408b-e21f-8aef-b65680000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "<strong>Cloning</strong> Cloning strategy is designed jointly by the user and the facility. Directional or non-directional cloning into any vector is performed by including right restriction sites in the primers, if necessary, for amplifying the insert for PCR-based cloning. After cloning the insertions sites are checked and orientations are determined by either restriction digestion and/or sequencing. PCR products are cloned using Invitrogen's TA cloning system or any other vector as requested. <strong>Sub-cloning</strong> After designing the cloning strategy, the insert is retrieved from the donor vector and appropriate restriction sites are created if necessary. The insert is then cloned into appropriate recipient vector followed by the screening for correct clones by restriction digestion and/or sequencing. We also offer ligation-independent cloning (LIC) when requested by the user."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/dnaseq/pricing.shtml#molbio
  4. Service Provided by
    DNA Sequencing Facility (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/dnaseq/cloning.shtml
  6. Related Technique
    Recombinant plasmid cloning
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2016-04-22T20:37:57.566-04:00
  4. creator
    fcoldren
  5. modified
    2016-04-22T20:38:12.602-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016