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Kspcre VHLf/f ICNotch1 mice

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000016c-67ad-82d9-b1cb-616580000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    Mice with TEC-specific overexpression of NOTCH1 intracellular domain and deletion of VHL were achieved by crossing the Kspcre mice (kindly provided by Dr. Igarashi, UTSW) with Gt(ROSA)26Sortm1(NOTCH1)Dam mice (obtained from Jackson Laboratory, stock no. 008159) and VHL floxed mice (Vhltm1Jae, obtained from The Jackson Laboratory, stock no. 004081). The major role of Jag1 and Jag2 is to activate Notch signaling. It has been difficult to express active Jagged1 on cell surface in vivo that would lead to Notch activation. This is mostly because following ligand presentation, Jagged1 is endocytosed with Notch extracellular domain. For these reasons, expression of Notch intracellular domain is the most accepted way to examine the role and effect of Notch signaling in vivo. Animals were identified by tail genomic PCR analysis. Male 8-week-old Kspcre VHLf/f ICNotch1 mice and Kspcre VHLf/f littermate controls were created.
  2. Contact
    Susztak, Katalin., MD, PhD
  3. Related Disease
    kidney cancer
  4. Related Publication or Documentation
    Notch Pathway Is Activated via Genetic and Epigenetic Alterations and Is a Therapeutic Target in Clear Cell Renal Cancer
  5. Parental Strain Name
    Kspcre mice
  6. Genetic Alteration(s)
    0000016c-67b5-8e3e-b1cb-616580000000
  7. Location
    0000016c-679c-ed01-b1cb-616580000000
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    ggrant (Gregory Grant)
  3. created
    2019-08-06T12:21:08.317-04:00
  4. creator
    ggrant (Gregory Grant)
  5. modified
    2020-02-13T10:12:54.772-05:00
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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016