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Recombinant plasmid DNA engineering
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000016d-8cf5-5af8-237e-a0bb80000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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"The facility staff will assist users in designing a cloning strategy to engineer an appropriate recombinant plasmid DNA that meets the specific goals of the project. The facility supports conventional, ligation independent, Gateway, and site-directed mutagenesis cloning technologies. The nucleic acids (e.g., cDNA, promoter, shRNA, etc.) are isolated from a customer-supplied plasmid DNA(s) and subcloned into a new vector of choice. Newly derived plasmid DNAs are authenticated by restriction endonuclease digestion, DNA sequencing, and prepared for subsequent transfection based applications."
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Service Fee URL
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https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
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Service Provided by
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Molecular Screening and Protein Expression Facility (Wistar)
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Website(s)
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https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
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Related Technique
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Plasmid construction
