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Recombinant plasmid DNA engineering

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000016d-8cf5-5af8-237e-a0bb80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The facility staff will assist users in designing a cloning strategy to engineer an appropriate recombinant plasmid DNA that meets the specific goals of the project. The facility supports conventional, ligation independent, Gateway, and site-directed mutagenesis cloning technologies. The nucleic acids (e.g., cDNA, promoter, shRNA, etc.) are isolated from a customer-supplied plasmid DNA(s) and subcloned into a new vector of choice. Newly derived plasmid DNAs are authenticated by restriction endonuclease digestion, DNA sequencing, and prepared for subsequent transfection based applications."
  3. Service Fee URL
    https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
  4. Service Provided by
    Molecular Screening and Protein Expression Facility (Wistar)
  5. Website(s)
    https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
  6. Related Technique
    Plasmid construction
 
RDFRDF
 
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016