Recombinant plasmid DNA engineering

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The facility staff will assist users in designing a cloning strategy to engineer an appropriate recombinant plasmid DNA that meets the specific goals of the project. The facility supports conventional, ligation independent, Gateway, and site-directed mutagenesis cloning technologies. The nucleic acids (e.g., cDNA, promoter, shRNA, etc.) are isolated from a customer-supplied plasmid DNA(s) and subcloned into a new vector of choice. Newly derived plasmid DNAs are authenticated by restriction endonuclease digestion, DNA sequencing, and prepared for subsequent transfection based applications."

3. Service Fee URL

   https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility

4. Service Provided by

   Molecular Screening and Protein Expression Facility (Wistar)

5. Website(s)

   https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility

6. Related Technique

   Plasmid construction