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Recombinant protein production in prokaryotic systems

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000016d-8cf7-1411-237e-a0bb80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Use of prokaryotic expression systems offers an economical method to achieve production of large amounts of recombinant protein. The Facility provides technical support for production of recombinant protein in bacteria. The core maintains a repository of inducible bacterial expression vector technologies. This collection of expression vectors allows for fusion of epitope tags (alone or in combination) to the protein of interest (GOI), including GST (Glutathione-S-transferase), 6 histidines (6His), SUMO, FLAG, or maltose binding protein (MBP) at the NH3- or COOH-terminus of a protein. High level protein expression (analytical or preparative scale) is achieved by IPTG induction of T7/T5 RNA polymerases in high-performance Epicuran Coli (e.g. Rosetta (DE3))." Services include: optimization of soluble protein expression and preparative production or recombinant proteins.
  3. Service Fee URL
    https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
  4. Service Provided by
    Molecular Screening and Protein Expression Facility (Wistar)
  5. Website(s)
    https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
  6. Related Technique
    Recombinant protein production
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    ggrant (Gregory Grant)
  3. created
    2019-10-02T10:56:12.842-04:00
  4. creator
    ggrant (Gregory Grant)
  5. modified
    2019-10-02T10:56:16.924-04:00
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016