Recombinant protein production via Baculovirus expression systems (BVES)

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "Baculovirus expression systems (BVES) represent an alternative approach to produce large amounts of properly folded and functional recombinant proteins. The benefits of protein expression with baculovirus include the virus being able to accommodate large inserts, eukaryotic post-translational modification, enhanced protein folding and function, high expression levels, easy scale up with high-density suspension culture, and safety. The Facility has >20 years of experience in preparing baculovirus vectors from nearly all commercially available BVES. Investigators have the option to provide the facility with previously constructed baculovirus transfer vectors, have facility staff engineer the plasmid DNA construct, or to provide the facility with small volumes of previously prepared virus stock." Available services include: preparation of high-titer baculovirus stock, amplification of high-titer baculovirus stocks, analytical scale productions for optimization of protein expression, and preparative scale productions."

3. Service Fee URL

   https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility

4. Service Provided by

   Molecular Screening and Protein Expression Facility (Wistar)

5. Website(s)

   https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility

6. Related Technique

   Recombinant protein production