Embryo Cryopreservation

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Cryopreservation provides a useful tool for archiving mouse lines. The reasons to cryopreserve strains include: -- Protect against the loss of valuable strains through breeding failure, disease, human error, etc. -- Eliminate the cost of maintaining inactive mouse lines. -- Free up space for other mouse lines. -- Facilitate future rederivation into a new facility. Frozen embryos can be stored indefinitely and thawed many years later, should the investigator require the use of the archived mouse line. Archiving a complex, involves more than two mutant alleles, mouse line is accomplished by freezing a minimum of 250 embryos. This method necessitates the use of 10-20 of your young male mice mated, overnight, with 10-20 of your female mice. The following day, viable 2-cell embryos are isolated and cryopreserved. The straws, containing the frozen embryos, are held in the Core large storage unit until needed. Viability of your cryopreserved line is performed by thawing a sample of embryos and culturing to the blastocyst stage. If requested, we can also surgically transfer some of the thawed embryos to generate live mice, but this would involve additional service fees.

3. Service Fee URL

   https://corelabs.research.chop.edu/sites/default/files/inline-files/Transgenic_Fee_Schedule_9.25.19.pdf

4. Service Provided by

   Transgenic Core (CHOP)

5. Website(s)

   https://corelabs.research.chop.edu/transgenic/services