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Sperm Cryopreservation

eagle-i ID


Resource Type

  1. Material production service


  1. Fee for service
  2. Resource Description
    Cryopreservation provides a useful tool for archiving mouse lines. The reasons to cryopreserve strains include: -- Protect against the loss of valuable strains through breeding failure, disease, human error, etc. -- Eliminate the cost of maintaining inactive mouse lines. -- Free up space for other mouse lines. -- Facilitate future rederivation into a new facility. Frozen sperm can be stored indefinitely and thawed many years later, should the investigator require the use of the archived mouse line. The Core will cryopreserve 15 samples (straws) of sperm for each strain. To achieve this, you must supply 3 healthy male mice, minimum of 12 weeks old, the mice will need to be individually housed at least 4 days prior to the scheduled freeze date. The sperm from the males is pooled together for the freezing. If necessary we can freeze the sperm from each male separately, but this will incur additional fees. There are two options for freezing sperm: without in-vitro fertilization, or with in-vitro fertilization. Sperm Cryopreservation (without IVF quality control) Approximately 2 days post-sperm freezing, a sample is retrieved from the liquid nitrogen unit, it is thawed, incubated, and used to assess the viability of the strain. The viability of the line is recorded and relayed to your lab. If the viability of the sperm is extremely low, a second session of freezing will be required to safely secure the line. However, it is not often that this is needed. Sperm Cryopreservation (with IVF quality control) Approximately a week post-freezing a single straw of the sperm is used for IVF, with wild-type oocytes. The following day viable zygotes are transferred into surrogate mother mice to reestablish the mouse line. When the offspring reach 10-14 days old they will be tail snipped, the tissues will be delivered to your lab and screened for the appropriate genotype. Mice identified with the correct genotype can be transferred to your animal holding room for future use, or euthanized.
  3. Service Fee URL
  4. Service Provided by
    Transgenic Core (CHOP)
  5. Website(s)
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    ggrant (Gregory Grant)
  3. created
  4. creator
    ggrant (Gregory Grant)
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016