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Genetic Manipulation

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000016d-df42-3dce-8bc3-757280000000

Resource Type

  1. Material modification service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    Most agents can be injected in the first forming cell with a fine glass needle and are then passed along to the dividing cells. CRISPR/Cas9 knock-outs are efficient and the core can create a complete knock out of a locus in the injected generation for most genes. Transgenic expression and integration is also efficient due to special tools. Most zebrafish researchers use the gateway cloning system that relies on 3 fragments (e.g. promoter, gene, fluorescent tag) that is cloned in a transgenesis vector; the fragments are interchangeable and a large tool box of different promoters, genes, and labels is available.
  3. Service Fee URL
    https://corelabs.research.chop.edu/aquatic-zebrafish/tools-fees
  4. Service Provided by
    Aquatic Zebrafish Core Laboratory (CHOP)
  5. Website(s)
    https://corelabs.research.chop.edu/aquatic-zebrafish
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    ggrant (Gregory Grant)
  3. created
    2019-10-18T10:26:41.179-04:00
  4. creator
    ggrant (Gregory Grant)
  5. modified
    2019-10-18T10:26:44.945-04:00
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016