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Genetic Manipulation

eagle-i ID


Resource Type

  1. Material modification service


  1. Fee for service
  2. Resource Description
    Most agents can be injected in the first forming cell with a fine glass needle and are then passed along to the dividing cells. CRISPR/Cas9 knock-outs are efficient and the core can create a complete knock out of a locus in the injected generation for most genes. Transgenic expression and integration is also efficient due to special tools. Most zebrafish researchers use the gateway cloning system that relies on 3 fragments (e.g. promoter, gene, fluorescent tag) that is cloned in a transgenesis vector; the fragments are interchangeable and a large tool box of different promoters, genes, and labels is available.
  3. Service Fee URL
  4. Service Provided by
    Aquatic Zebrafish Core Laboratory (CHOP)
  5. Website(s)
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    ggrant (Gregory Grant)
  3. created
  4. creator
    ggrant (Gregory Grant)
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016