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KI MICE – LARGE SEQUENCES
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000016e-18b8-c385-2fb3-943680000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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Generation of knock-in (KI) mice containing large sequences (e.g. fluorescent proteins, STOP cassettes) usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNA, preparation of donor dsDNA templates, microinjection of one-cell embryos, and screening of founder mice.
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Service Fee URL
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https://pathbio.med.upenn.edu/crispr/site/fees/
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Service Provided by
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CRISPR/Cas9 Mouse Targeting Core
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Website(s)
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https://pathbio.med.upenn.edu/crispr/site/services/
