KI MICE – LARGE SEQUENCES

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Generation of knock-in (KI) mice containing large sequences (e.g. fluorescent proteins, STOP cassettes) usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNA, preparation of donor dsDNA templates, microinjection of one-cell embryos, and screening of founder mice.

3. Service Fee URL

   https://pathbio.med.upenn.edu/crispr/site/fees/

4. Service Provided by

   CRISPR/Cas9 Mouse Targeting Core

5. Website(s)

   https://pathbio.med.upenn.edu/crispr/site/services/