Ultracentrifugation (UC): Extracellular Vesicle Isolation

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   UC is used for initial separation of EVs from biofluid through differential and sequential spins, including a 20,000 x g spin for larger microvesicles and concluding with a high-speed spin for those isolating “small EVs” or “exosomes” (30-150nm in size). After getting your initial EV pellet (whether high speed or low speed), UC can also be used to create a density gradient, to separate your EVs from any non-vesicular contaminating factors in your pellet.

3. Service Fee URL

   https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services

4. Service Provided by

   Extracellular Vesicle Core

5. Website(s)

   https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services