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Ultracentrifugation (UC): Extracellular Vesicle Isolation
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000016e-8029-cc05-2da7-47fb80000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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UC is used for initial separation of EVs from biofluid through differential and sequential spins, including a 20,000 x g spin for larger microvesicles and concluding with a high-speed spin for those isolating “small EVs” or “exosomes” (30-150nm in size). After getting your initial EV pellet (whether high speed or low speed), UC can also be used to create a density gradient, to separate your EVs from any non-vesicular contaminating factors in your pellet.
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Service Fee URL
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https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services
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Service Provided by
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Extracellular Vesicle Core
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Website(s)
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https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services
