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Ultracentrifugation (UC): Extracellular Vesicle Isolation

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    UC is used for initial separation of EVs from biofluid through differential and sequential spins, including a 20,000 x g spin for larger microvesicles and concluding with a high-speed spin for those isolating “small EVs” or “exosomes” (30-150nm in size). After getting your initial EV pellet (whether high speed or low speed), UC can also be used to create a density gradient, to separate your EVs from any non-vesicular contaminating factors in your pellet.
  3. Service Fee URL
  4. Service Provided by
    Extracellular Vesicle Core
  5. Website(s)
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    ggrant (Gregory Grant)
  3. created
  4. creator
    ggrant (Gregory Grant)
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016