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Ultracentrifugation (UC): Extracellular Vesicle Isolation

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000016e-8029-cc05-2da7-47fb80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    UC is used for initial separation of EVs from biofluid through differential and sequential spins, including a 20,000 x g spin for larger microvesicles and concluding with a high-speed spin for those isolating “small EVs” or “exosomes” (30-150nm in size). After getting your initial EV pellet (whether high speed or low speed), UC can also be used to create a density gradient, to separate your EVs from any non-vesicular contaminating factors in your pellet.
  3. Service Fee URL
    https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services
  4. Service Provided by
    Extracellular Vesicle Core
  5. Website(s)
    https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core/evc-services
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    ggrant (Gregory Grant)
  3. created
    2019-11-18T15:20:32.712-05:00
  4. creator
    ggrant (Gregory Grant)
  5. modified
    2019-11-18T15:20:36.611-05:00
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016