Tissue Culture and Antibody Service

eagle-i ID

Resource Type

Properties

1. Resource Description

   1) Generation of a variety of cell culture models: a) Primary cultures of neurons, astrocytes, microglia and oligodendrocytes b) Co-cultures of specific cell types c) Organotypic cultures 2) Culture maintenance and use: a) Propagation, amplification and cryopreservation of these cultures. b) Variety of purification methods: immunopanning, "shake-off", Macs manual cell sorting c) Evaluation of other culture and cell separation methods such as adult neural tissue cell separator (coming soon). d) After exposure to agents: monitoring of cell death, toxicity, proliferation, differentiation, morphological alterations 3) Antibody services: a) Validation and use of specific antibodies for neural cells

2. Contact

   Grinspan, Judith., PhD

3. Service Provided by

   Preclinical Models Core

4. Website(s)

   https://iddrc.research.chop.edu/cores_pmc.php