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Tissue Culture and Antibody Service
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000016e-f161-fb8b-396c-07d780000000
Resource Type
Properties
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Resource Description
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1) Generation of a variety of cell culture models:
a) Primary cultures of neurons, astrocytes, microglia and oligodendrocytes
b) Co-cultures of specific cell types
c) Organotypic cultures
2) Culture maintenance and use:
a) Propagation, amplification and cryopreservation of these cultures.
b) Variety of purification methods: immunopanning, "shake-off", Macs manual cell sorting
c) Evaluation of other culture and cell separation methods such as adult neural tissue cell separator (coming soon).
d) After exposure to agents: monitoring of cell death, toxicity, proliferation, differentiation, morphological alterations
3) Antibody services:
a) Validation and use of specific antibodies for neural cells
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Contact
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Grinspan, Judith., PhD
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Service Provided by
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Preclinical Models Core
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Website(s)
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https://iddrc.research.chop.edu/cores_pmc.php
