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A single copy of Huygens Essential deconvolution/ image restoration software by Scientific Volume Imaging is available for use with Leica LIF files on the workstation computer in the core manager’s office. This software can be used on image stacks taken on the core’s Leica DM6000B widefield epifluorescence microscope to remove out of focus haze from the images to yield almost confocal quality images. Image stacks taken with the core’s Leica SP5 or SP8 confocal microscopes can be deconvolved to yield super-resolution images, provided image acquisition was optimized to make the most of the available optical resolution.
Penn Vet Imaging Core