The Nucleic Acid/Protein Research Core Facility, located on the 9th floor of the Abramson Building, provides a centralized source for specialized services, technical expertise and reagents to support investigators' molecular biology research needs. These services include DNA sequencing analysis, fluorescent fragment analysis (microsatellite genotyping), oligonucleotide synthesis, microarray services, denaturing high-performance liquid chromatography, Affymetrix GeneChip expression analysis, SNP analysis and RNA analysis.
"The 3730 DNA analyzer is a 48-capillary instrument capable of performing DNA sequencing analysis, fragment analysis (formerly called GeneScan) and SNP analysis."
"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated, high quality digital data.
Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows. Launched in 1999, the Agilent 2100 Bioanalyzer was the first commercially available instrument to use microfluidics technology for the analysis of biological samples. Today, the Bioanalyzer offers a broad range of pre-validated analysis kits combined with an easy-to-use benchtop system."
"BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems is used for sequencing. Sequencing products are analyzed on a 3730 DNA Analyzer from Applied Biosystems."
"There are two formats to submit DNA sequencing requests and the prices are different:"
• premixed: DNA template and primer are submitted premixed in tube
• traditional: DNA template and primer are submitted in separate tubes
NAPCore provides standard primers
Oligonucleotides are purified by high-performance liquid chromatography (HPLC) or polyacrylamide gel electrophoresis (PAGE). Pricing depends on the quantity of oligonucleotide to be purified (100 nmol, 250 nmol, or 1000nmol).
"This is a collaboration between CHOP's Nucleic Acid/Protein Research Core Facility (NAPCORE) and IDT. NAPCORE customers can order their oligo directly from IDT through this site, but must physically go to NAPCORE to pick up oligos."