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Histology and Gene Expression Core (Penn)

Director: Lu, Min Min

Summary:

The Histology and Gene Expression Core Facility provides expert professional services for members of the Cardiovascular Institute. Services for non- Institute investigators are available on a fee-for-service basis as time permits. The Histology and Gene Expression Core offers all histology-related services include tissue processing, embedding, sectioning, staining, immunocytochemistry and InSitu Hybridization.

Affiliations:

People:

Resources:

Protocols

Reagents

  • anti-actin ( Monoclonal antibody reagent )

    "The antibody is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections."

  • anti-beta galactosidase ( Polyclonal antibody reagent )

  • anti-CD31 ( Monoclonal antibody reagent )

  • anti-P-ERK ( Polyclonal antibody reagent )

    "Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when phosphorylated either individually or dually at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2). The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP Kinase, and does not cross-react with non-phosphorylated Erk1/2."

  • Goat anti-GFP ( Polyclonal antibody reagent )

    Goat polyclonal to GFP recognizes wild type, recombinant and enhanced forms of GFP.

Services

  • Immunohistochemistry (IHC) staining service ( Material processing service )

    Paraffin slides and frozen slides can be submitted for IHC staining. The user selects the primary antibody (with dilution) and detection method. Antibodies can be selected from antibodies available in the core or submitted by the user.

    Generally, the IHC slides will be ready in one or two weeks.

  • Staining service ( Material processing service )

  • Tissue processing and sectioning service ( Material processing service )


Web Links:

Last updated: 2016-06-14T15:26:42.190-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016