The Histology and Gene Expression Core Facility provides expert professional services for members of the Cardiovascular Institute. Services for non- Institute investigators are available on a fee-for-service basis as time permits. The Histology and Gene Expression Core offers all histology-related services include tissue processing, embedding, sectioning, staining, immunocytochemistry and InSitu Hybridization.
Staining protocols include:
• H&E stain
• McMannus' Periodic Acid Schiff's (PAS) stain
• Verhoeff-Van Gieson (Elastin) stain
• Masson's Trichrome Stain
• Modified Movat's Pentachrome stain
Procedure for alkaline phosphatase and horseradish peroxidase immunostaining using reagents from Vector Laboratories.
The protocols are for tissue sample processing for subsequent light microscopy with H&E staining, immunocytochemistry or in situ hybridization.
Includes procedures for:
• general tissue
• embryo fixation
• aortic arch and heart (starting from dissection)
"There are two kind of frozen tissue which are used for immunocytochemistry: fresh frozen tissue and fixed frozen tissue. The fresh frozen tissue will be frozen immediately after the animal was dissected. The fixed frozen tissue should be fixed first then frozen."
"The antibody is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections."
"Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when phosphorylated either individually or dually at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2). The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP Kinase, and does not cross-react with non-phosphorylated Erk1/2."
Goat polyclonal to GFP recognizes wild type, recombinant and enhanced forms of GFP.
Paraffin slides and frozen slides can be submitted for IHC staining. The user selects the primary antibody (with dilution) and detection method. Antibodies can be selected from antibodies available in the core or submitted by the user.
Generally, the IHC slides will be ready in one or two weeks.