The Human Pluripotent Stem Cell Core was established in 2008 as part of the Center for Cellular and Molecular Therapeutics. Our mission is to provide expertise and quality-control reagents for the culture and differentiation of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) to CHOP, the University of Pennsylvania, and outside academic communities.
An infrastructure and solid foundation has been created for the generation of iPSC lines that are used by investigators worldwide for modeling human disease to study mechanism, development, and establish new therapeutic modalities. A recent addition to the core has been the establishment of the genome editing technology using CRISPR/Cas. This technology is being utilized on iPSCs established by the Core for creating isogenic lines that eliminate the clonal heterogeneity and variability in downstream applications. We provide enrichment courses to actively train investigators with the necessary tools and expertise to maximize successful outcomes.
Propagate and distribute human PSC lines to investigators
Differentiate human PSCs into the three germ layers and their derivative tissues