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Translational Biomarker Core (Penn)

Director: Blair, Ian A., Ph.D.

Summary:

This core provides sophisticated analytical services based on liquid chromatography-mass spectrometry.

Affiliations:

People:

Resources:

Instruments

  • Agilent 6460 triple quadrupole mass spectrometer with Agilent 1200 HPLC system ( Liquid chromatography mass spectrometry platform )

    The Agilent 6460 is equipped with an Agilent 1200 series LC system.

    "The 6460 Triple Quad LC/MS is a feature-packed new addition to the Agilent Triple Quadrupole product family:
    • excellent sensitivity for many applications
    • 1 ms dwell time with no collision cell crosstalk
    • very fast polarity switching for multi-analyte studies
    • dynamic Multiple Reaction Monitoring (dMRM) to maximize MRM efficiency
    • triggered Multiple Reaction Monitoring (tMRM) for simultaneous quantitation and confirmation"

  • Thermo Scientific LTQ-XL ion trap mass spectrometer with Eksigent NanoLC system ( Liquid chromatography mass spectrometry platform )

    "Developed specifically for nanospray mass spectrometry (MS), Eksigent NanoLC systems provide speed, sensitivity, resolution and reliability for your research."

    "Thermo Scientific LTQ XL high performance linear ion trap delivers more structural information than any other ion trap mass spectrometer. LTQ XL is the only mass spectrometer that offers multiple dissociation techniques, PQD, ETD and CID. Pulsed Q Collision Induced Dissociation (PQD) is a proprietary technique that eliminates the low mass cut-off concern inherent with all ion traps. This results in extensive coverage for predicted and unpredicted metabolites, and the ability to perform peptide quantification using iTRAQ* labels. In addition, fast polarity switching for high-sensitivity analysis of unknowns, high resolution isolation (HRI), intelligent Data Dependent acquisition, and optional high resolution accurate mass functionality are all tools to help the researcher gain confident structural identification routinely."

    Two LTQ-XL are available.

  • Thermo Scientific TSQ Vantage triple quadrupole mass spectrometer with Waters nanoAcquity UPLC system ( Liquid chromatography mass spectrometry platform )

    "Quantify small molecules, peptides, biosimilars, and biologics using the Thermo Scientific™ TSQ Vantage™ Triple Quadrupole Mass Spectrometer. With high sensitivity and low noise, the TSQ Vantage system delivers high assay precision and accuracy for a range of bioanalytical, clinical research, environmental, and proteomics applications. It includes the Thermo Scientific™ Ion Max™ ion source for dependable, easy operation."

  • Thermo TSQ Quantum Ultra triple quadrupole mass spectrometer with Waters 2690 Alliance series HPLC system ( Liquid chromatography mass spectrometry platform )

    "Key Features of the TSQ:
    • Ion Max source with the HESI-II heated electrospray ionization probe
    • Unmatched sensitivity and specificity with H-SRM
    • Supports 21 CFR Part 11 compliance through Thermo Scientific LCQUAN software
    • Extended Mass Range (EMR) and Accurate Mass (AM) models available

    Breakthrough innovations such as H-SRM, Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS), and options such as Thermo Scientific Transcend system powered by proprietary TurboFlow* technology, and high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) facilitate the development of robust quantitative methods and provide a significant reduction in matrix interferences."

Services

  • Biomarker quantification by mass spectrometry ( Material analysis service )

    "Rigorous quantification of serum, plasma or urinary biomarkers identified by metabolomics studies can be conducted using stable isotope dilution LC-SRM/MS methodology as described by Ciccimaro and Blair (Bioanalysis. 2010;2:311-341)."

Software

  • MassHunter ( Software )

    "The all new Agilent MassHunter Workstation software provides intuitive, yet powerful, instrument control, data acquisition, qualitative and quantitative data analysis, and reporting for Agilent time-of-flight (TOF), quadrupole time-of-flight (Q-TOF), ICP-MS, and triple quadrupole systems. Designed from the ground up to make MS analyses easier from tuning to final report MassHunter Workstation software can be complemented by application-specific MassHunter software packages that provide even more power and stream-lined operation for specialized analytical tasks such as expression profiling."


Last updated: 2016-05-16T12:52:11.088-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016