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Blair Laboratory

Summary:

Research in our laboratory is heavily involved in the use of mass spectrometry for proteomics, lipidomics, and DNA analysis. We are particularly interested in determining the factors that control lipid hydroperoxide-mediated damage to DNA, RNA, and proteins. Methodology is being developed to characterize covalent modifications to these macromolecules using novel mass spectrometry techniques, determining how these can be evaluated as potential “biomarkers” of various physiological processes and disease states, and assessing how such processes can be prevented using novel pharmacological agents.

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Instruments

  • Applied Biosystems API4000 triple quadrupole mass spectrometer with Agilent 1100 HPLC system ( Liquid chromatography mass spectrometry platform )

    The API4000™ system, equipped with an Agilent 1100 Series LC system, has the following features:
    • detector: pulse counting CEM
    • ionization sources: TurbolonSpray, APCI, DuoSpray, and PhotoSpray
    • scan types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM

  • Thermo Scientific LTQ ion trap mass spectrometer with Waters 2690 Alliance HPLC system ( Ion trap mass spectrometer )

  • Thermo TSQ Quantum Ultra triple quadrupole mass spectrometer with Waters 2690 Alliance HPLC system ( Liquid chromatography mass spectrometry platform )

    "Key Features of the TSQ:
    • Ion Max source with the HESI-II heated electrospray ionization probe
    • Unmatched sensitivity and specificity with H-SRM
    • Supports 21 CFR Part 11 compliance through Thermo Scientific LCQUAN software
    • Extended Mass Range (EMR) and Accurate Mass (AM) models available

    Breakthrough innovations such as H-SRM, Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS), and options such as Thermo Scientific Transcend system powered by proprietary TurboFlow* technology, and high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) facilitate the development of robust quantitative methods and provide a significant reduction in matrix interferences."


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Last updated: 2013-02-26T12:46:31.558-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016