Research in our laboratory is heavily involved in the use of mass spectrometry for proteomics, lipidomics, and DNA analysis. We are particularly interested in determining the factors that control lipid hydroperoxide-mediated damage to DNA, RNA, and proteins. Methodology is being developed to characterize covalent modifications to these macromolecules using novel mass spectrometry techniques, determining how these can be evaluated as potential “biomarkers” of various physiological processes and disease states, and assessing how such processes can be prevented using novel pharmacological agents.
The API4000™ system, equipped with an Agilent 1100 Series LC system, has the following features:
• detector: pulse counting CEM
• ionization sources: TurbolonSpray, APCI, DuoSpray, and PhotoSpray
• scan types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
"Key Features of the TSQ:
• Ion Max source with the HESI-II heated electrospray ionization probe
• Unmatched sensitivity and specificity with H-SRM
• Supports 21 CFR Part 11 compliance through Thermo Scientific LCQUAN software
• Extended Mass Range (EMR) and Accurate Mass (AM) models available
Breakthrough innovations such as H-SRM, Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS), and options such as Thermo Scientific Transcend system powered by proprietary TurboFlow* technology, and high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) facilitate the development of robust quantitative methods and provide a significant reduction in matrix interferences."