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The Genotyping and DNA/RNA Analysis Core (Monell)

Director: Reed, Danielle., PhD

Summary:

This Research Core provides training and research support in genotyping and quantification of nucleic acids. The Core facility has equipment needed to genotype DNA samples and to measure nucleic acid concentrations in isolation or in tissue samples.

Affiliations:

People:

Resources:

Instruments

  • ABI Prism 7300 ( Real-time PCR machine )

    "The 7300 Real-Time PCR System balances exceptional quality with budget sensitivity. Advanced multicolor detection capabilities allow you to perform a wide variety of applications with the highest confidence in your results.

    Applications include gene expression analysis, absolute quantitation, SNP genotyping, and +/– assays utilizing internal positive controls. The 7300 System is optimized for use with standard dye sets: FAMTM/SYBR® Green I, VIC®/JOETM, NEDTM, TAMRATM, and ROXTM."

  • ABI PRISM® 7000 ( Real-time PCR machine )

    "The ABI PRISM® 7000 Sequence Detection System (7000 SDS instrument) is a second- generation sequence detection instrument capable of quantitative and qualitative detection with fluorescent-based PCR chemistries. The instrument is capable of quantitative detection using real-time analysis, and qualitative detection using end-point and dissociation-curve analysis.

    The 7000 SDS instrument combines thermal cycling, fluorescence detection, and application-specific software. It detects accumulated polymerase chain reaction (PCR) product cycle-by-cycle, thus making quantification available immediately after completion of PCR, without the need for further process analysis."

    excerpt from Getting Started Guide, p1.

  • ABI StepOne™ ( Real-time PCR machine )

  • ABI7000 ( DNA sequencer )

  • Applied Biosystems Veriti ( Thermal cycler )

    "The Veriti® Thermal Cycler features powerful innovations for precise control of your PCR optimization, including a color touch-screen and intuitive interface, plus VeriFlex™ Blocks—six independent temperature blocks—for increased flexibility and precision. The easy-to-use touch-screen provides a simple yet powerful user interface accelerating both instrument setup and use. The optional VeritiLink® Remote Management Software allows email updates on cycler status.
    The Veriti® Thermal Cycler is available in four formats:
    • 96-well, 0.1 mL VeriFlex™ Block
    • 96-well, 0.2 mL VeriFlex™ Block
    • 384-well, 0.02 mL block
    • 60-well, 0.5 mL block"

  • Eppendorf MasterCycle gradient machine ( Instrument )

  • Nanodrop 1000 spectrophotometer ( Spectrophotometer )

    "The Thermo Scientific NanoDropTM 1000 Spectrophotometer measures 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer).

    Applications
    • Nucleic acid concentration and purity of nucleic acid samples up to 3700 ng/ul (dsDNA) without dilution
    • Fluorescent dye labeling density of nucleic acid microarray samples
    • Purified protein analysis (A280) up to 100 mg/ml (BSA)
    • Expanded spectrum measurement and quantitation of fluorescent
    dye labeled proteins, conjugates, and metalloproteins
    • Bradford Assay analysis of protein
    • BCA Assay analysis of protein
    • Lowry Assay analysis of protein
    • Pierce Protein 660 nm Protein Assay
    • Cell density measurements
    • General UV-Vis spectrophotometry"

    excerpt from NanoDrop 1000 Spectrophotometer v3.7 User's Manual


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Last updated: 2016-05-10T17:31:33.263-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016