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Stem Cell & Xenograft Core (Penn)

Directors: Carroll, Martin, M.D.; Danet-Desnoyers, Gwenn, Ph.D.; Shan, Xiaochuan, MD, PhD

Summary:

The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory that integrates a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies with a full range of xenograft services.

The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues. Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available. For consultation or questions, please contact Martin Carroll.

The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space. For consultation or questions, please contact Gwenn Danet-Desnoyers.

Pennkey required to request services, contact to acquire a guest Pennkey if needed.

Affiliations:

People:

Resources:

Biological Specimens

Databases

  • Hematopoietic malignancy tissue bank database ( Database )

    "We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells."

Instruments

Organisms and Viruses

  • BLT mice ( Mus musculus )

    "The BLT mouse is a modification of the SCID-Hu model which was developed by Joseph McCune in the early nineties. To construct the BLT model, NOD/SCID mice are cotransplanted with human liver and thymus tissues along with autologous CD34+ hematopoietic stem cells. Development of a robust human hemato-lymphoid system is observed between 12-16 weeks post engraftment with human cells and tissues. This model is also being used extensively in HIV research and studying of virus-specific human immune responses. The BLT mice are, however, susceptible to thymic lymphoma and may not allow long term studies (average lifespan ~8.5 months)."

  • Human immune system NSG mice ( Mus musculus )

    "We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV."

  • Immune-deficient (NSG) mice ( Mus musculus )

    "The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies."

  • Jax NSG mice ( Mus musculus )

  • Jax NSGS mice ( Mus musculus )

    "The triple transgenic NSG-SGM3 (NSGS) mice expressing human IL3, GM-CSF and SCF combine the features of the highly immunodeficient NOD scid gamma (NSG) mouse with cytokines that support the stable engraftment of myeloid lineages and regulatory T cell populations. As a result, these NSG-SGM3 mice allow superior engraftment of diverse hematopoietic lineages and primary AML samples than other models and are useful for immuno-oncology, immunology and infectious disease studies."

  • NOD-Rag1 null IL2Rgc null mice ( Mus musculus )

  • NSG mice expressing human NSG-S, GM-CSF and IL-3 ( Mus musculus )

Services

  • AutoMACS access ( Access service )

    Use of AutoMACS Immunomagnetic Selection Device

  • AutoMACS training service ( Training service )

  • Banking techniques and xenograft training ( Service )

    "Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available."

    "The SCXC offers a wide variety of xenograft services from training to full-service experiments."

  • Cell banking in LN2 freezer ( Material storage service )

  • Consultation services ( Support service )

    • project feasibility
    • model development
    • experimental design and planning
    • troubleshooting

  • Custom flow cytometry on clinical specimens ( Material processing service )

  • Custom immunomagnetic selection ( Material processing service )

  • Dedicated patient specimen-MNC ( Material maintenance service )

  • Ficoll processing service ( Material processing service )

  • Flow cytometry on mouse tissue ( Material analysis service )

    4 color

  • Patient specimen delivery from clinic to lab ( Transport service )

  • Patient Specimen-Custom identification, acquisition, unprocessed ( Material processing service )

  • Tissue bank access ( Access service )

    "Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells."

  • Xenograft services ( Material modification service )

    "The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space."

    Xenograft services include:
    Blood glucose measurement
    Busulfan Conditioning
    Injections (iv, ip, sc) in Mice
    Intra bone injection
    Intra Cranial injections
    Irradiation
    Mouse body scoring
    Mouse bone marrow aspirate
    Mouse health monitoring
    Mouse Weighting
    Necropsy service
    Oral Gavage
    Orthotopic liver injection
    Orthotopic ovarian injection
    Orthotopic Pancreatic injection
    Osmotic Pump Implant
    Oxivir
    Phlebotomy Samples
    Renal capsule injection
    Topical drug application
    Tumor Measurements


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Last updated: 2016-05-16T12:53:20.786-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016