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|  University of Pennsylvania |
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| University of Pennsylvania |
The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory that integrates a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies with a full range of xenograft services.
The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues. Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available. For consultation or questions, please contact Martin Carroll.
The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space. For consultation or questions, please contact Gwenn Danet-Desnoyers.
Pennkey required to request services, contact to acquire a guest Pennkey if needed.
• fresh
• frozen
• fresh
• frozen
• fresh
• frozen
• non pheresis
• NSG engrafting
• pheresis product
"We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells."
"located within the Core's BSL-2 space"
"The BLT mouse is a modification of the SCID-Hu model which was developed by Joseph McCune in the early nineties. To construct the BLT model, NOD/SCID mice are cotransplanted with human liver and thymus tissues along with autologous CD34+ hematopoietic stem cells. Development of a robust human hemato-lymphoid system is observed between 12-16 weeks post engraftment with human cells and tissues. This model is also being used extensively in HIV research and studying of virus-specific human immune responses. The BLT mice are, however, susceptible to thymic lymphoma and may not allow long term studies (average lifespan ~8.5 months)."
"We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV."
"The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies."
"The triple transgenic NSG-SGM3 (NSGS) mice expressing human IL3, GM-CSF and SCF combine the features of the highly immunodeficient NOD scid gamma (NSG) mouse with cytokines that support the stable engraftment of myeloid lineages and regulatory T cell populations. As a result, these NSG-SGM3 mice allow superior engraftment of diverse hematopoietic lineages and primary AML samples than other models and are useful for immuno-oncology, immunology and infectious disease studies."
Use of AutoMACS Immunomagnetic Selection Device
"Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available."
"The SCXC offers a wide variety of xenograft services from training to full-service experiments."
• project feasibility
• model development
• experimental design and planning
• troubleshooting
4 color
"Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells."
"The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space."
Xenograft services include:
Blood glucose measurement
Busulfan Conditioning
Injections (iv, ip, sc) in Mice
Intra bone injection
Intra Cranial injections
Irradiation
Mouse body scoring
Mouse bone marrow aspirate
Mouse health monitoring
Mouse Weighting
Necropsy service
Oral Gavage
Orthotopic liver injection
Orthotopic ovarian injection
Orthotopic Pancreatic injection
Osmotic Pump Implant
Oxivir
Phlebotomy Samples
Renal capsule injection
Topical drug application
Tumor Measurements
